Views:4 Author:Site Editor Publish Time: 2020-06-06 Origin:Site
There are so many types of blood collection tubes in the clinic, do you really know clearly? The different colors of the caps and labels of the vacuum collection heads mean that the types of additives in the blood collection tubes and the purpose of the test are different. Medical staff can choose the corresponding test tube according to the relevant requirements of the physical examination items or experimental indicators. At present, the common vacuum blood collection tubes in clinic are generally divided into the following types:
1. Ordinary serum tube (red cap), without any additives in the blood collection tube, usually about 10 minutes to coagulate the blood, mainly used for routine serum biochemical tests. Common components of the blood include the red blood cells, hemoglobin in the red blood cells, white blood cells, and platelets. There are more than 20 indicators.
2. Rapid procoagulant serum tube (orange-red head), blood collection tube contains procoagulant, activates fibrinase, makes soluble fibrin into insoluble fibrin polymer, and then forms stable fibrin clot. Generally, the blood is coagulated within 5 minutes, which is mainly used for emergency serum biochemical test.
3. Inert separation gel coagulation tube (golden yellow head cap), the blood collection tube contains inert separation gel and coagulant. The separation hose has good affinity and plays a role in isolation. After centrifuging the specimen, the separation rubber can completely separate the liquid components (serum) and solid components (blood cells) in the blood and accumulate in the test tube to form a barrier. The specimens remained stable for 48 hours under the condition of cold storage of serum. Mainly used for serum biochemistry (liver function, kidney function, myocardial enzymes, amylase, etc.), electrolytes (serum potassium, sodium, **, calcium, phosphorus, etc.), thyroid function, drug testing, AIDS testing, tumor markers, PCR , TORCH, serum immunological detection, using ELISA kits to detect various inflammatory factors (IL-2, IL-6, TNF-a, etc.).
4. Sodium citrate erythrocyte sedimentation test tube (black cap), the concentration of sodium citrate required for erythrocyte sedimentation test is 3.2% (equivalent to 0.109mol/L), the ratio of anticoagulant to blood is 1:4, general anti When the ratio of coagulant is too high, the blood is diluted, which can accelerate the erythrocyte sedimentation rate. When collecting blood, it is necessary to collect enough blood (2ml). After blood collection, it should be inverted and mixed 8-10 times immediately to ensure the accuracy of the test results.
5. Sodium citrate coagulation test tube (light blue head cap), sodium citrate in blood collection tube chelate with calcium ion in blood sample to play an anticoagulant effect, anticoagulant to blood ratio is 1:9, recommended anticoagulation The concentration of the agent is 3.8% (equivalent to 0.129mol/L), which is mainly used in the fibrinolytic system (prothrombin time, thrombin time, activated partial thrombin time, fibrinogen). When collecting blood, it is necessary to collect enough blood (2ml). After blood collection, it should be inverted and mixed 8-10 times immediately to ensure the accuracy of the test results.
6. Heparin anticoagulation tube (green cap), blood collection tube contains heparin. Heparin has a direct antithrombin effect, which can extend the clotting time of the specimen. Mainly suitable for erythrocyte fragility test, blood gas analysis, hematocrit test, erythrocyte sedimentation rate and general biochemical determination, etc.
7. Plasma separation tube (light green cap), heparin anticoagulant is added to the inert separation tube, which can achieve the purpose of rapid plasma separation. It is the best choice for electrolyte detection, and can also be used for routine plasma biochemical determination and ICU and other emergency departments Plasma biochemical testing. Plasma specimens remained stable for 48 hours under refrigeration.
8. Potassium oxalate/sodium sulfide blood collection tube (gray head), sodium sulfide is a weak anticoagulant, usually used in combination with potassium oxalate, the ratio is 1 part of sodium oxalate, oxalic acid 3 servings of potassium. This mixture of 4mg can prevent 1ml of blood from coagulating and inhibiting the decomposition of sugar in 23 days. It is an excellent preservative for blood glucose measurement. When using it, pay attention to invert and mix slowly. Note that it cannot be used for the determination of urea by the urease method, nor for the detection of alkaline phosphatase and amylase.
9. EDTA anticoagulation tube (purple head), the blood collection tube contains EDTA salt, which can effectively chelate calcium ions in blood samples, chelate calcium or remove the calcium reaction site, blocking Stagnation and termination of endogenous or exogenous clotting process, thereby preventing blood specimens from clotting. After centrifugation, the upper plasma is mainly suitable for general hematological tests, such as homocysteine, adenosyl homocysteine, and adenosylmethionine levels in plasma. It is not suitable for coagulation tests and platelet function tests.