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What are the additives in blood collection tubes?

Views:1     Author:Site Editor     Publish Time: 2021-05-11      Origin:Site

The additives in the blood collection tube include: anticoagulant, coagulant, buffer, protective agent, inner wall treatment agent, injection day treatment agent, separating glue and so on. Its variety, performance, and concentration directly affect the characteristics and detection of blood samples. The commonly used ones are as follows:


One, biochemical

Biochemical blood collection tubes are divided into additive-free tubes (red caps), coagulation promoting tubes (orange caps), and separation hoses (yellow caps). The inner wall of the high-quality additive-free blood collection tube is evenly coated with inner wall treatment agent and nozzle treatment agent to avoid cell breakage during centrifugation and affect the test results. The inner wall and serum of the tube are clear and transparent, and there is no blood on the nozzle. In addition to the inner wall of the coagulation tube being evenly coated with the inner wall treatment agent and the nozzle treatment agent, the inside of the tube is sprayed to make the coagulant evenly attached to the tube wall, which is convenient for quickly and fully mixing the blood sample after sampling, which can greatly shorten the clotting time. And no fibrin filaments are separated out to avoid clogging the pinholes of the equipment during sampling. When the separation tube is centrifuged, the separation gel is liquefied and transferred to the center of the tube, between the formation of serum or plasma and blood. After centrifugation, it solidifies and forms a barrier to completely separate the serum or plasma from the cells, ensuring the stability of the serum chemical composition , There is no obvious change in 48 h under refrigeration. The inert separation hose is filled with heparin, which can achieve the purpose of rapid plasma separation, and the sample can be stored for a long time. The above separation hose can be used for rapid biochemical determination. Separation gel heparin tube is suitable for emergency, acute pituitary ward ((ICU) and other biochemical tests. Compared with serum tube, the advantage is that the serum (plasma) separates quickly, and the second is that the chemical composition of serum (plasma) can be stable for a long time. Change, easy to transport.

Two, anticoagulant

1) Heparin tube (green cap): Heparin is an excellent anticoagulant, which has little interference to blood components, does not affect the volume of red blood cells, and does not cause hemolysis. It is suitable for red blood cell permeability test, blood gas, plasma permeability, red blood cell Pack volume, erythrocyte sedimentation rate and general biochemical determination.

2) Blood routine tube (purple cap): EDTA is superfluous with calcium ions in the blood, so that the blood does not coagulate. Generally, 1.0-2.0 mg can prevent 1ml of blood coagulation. This anticoagulant does not affect the white blood cell count and size, has the least effect on the morphology of red blood cells, and can inhibit the aggregation of platelets, which is suitable for general hematology tests. The spray method is usually adopted to make the reagents evenly adhere to the tube wall. It is convenient to quickly and fully mix the blood sample after sampling.

3) Blood coagulation tube (blue cap): a quantitative liquid sodium citrate anticoagulant buffer is added to the blood collection tube. The anticoagulant and the rated blood volume are added in a ratio of 1:9, which is used for the inspection of the coagulation mechanism project. Six (such as PT. APTT). The principle of anticoagulation is to combine with calcium to form a soluble calcium chelate to prevent blood from clotting. The recommended concentration of anticoagulant required for blood coagulation determination is 3.2% or 3.8%, which is equivalent to 0.109 or 0.129 mo1/L. For blood coagulation tests, if the blood ratio is too low, the APTT time will be prolonged, and the prothrombin time (PT) staining will also change significantly. Therefore, whether the ratio of anticoagulant to the rated blood volume is accurate. This is the decision An important standard for product quality.

4) Erythrocyte sedimentation tube (black cap): The anticoagulation system of blood collection tube is the same as that of blood coagulation tube, except that sodium citrate anticoagulant and the rated blood volume are added in a ratio of 1:4. It is used for erythrocyte sedimentation rate inspection.

5) Blood glucose tube (gray): The blood collection tube is added with fluoride as an inhibitor. The addition of r-inhibitor and the special treatment of the inner wall of the test tube make the original character of the blood sample to be maintained for a longer time. The blood cell metabolism is stagnant. Widely used J. Check blood sugar, glucose tolerance, erythrocyte electrophoresis, anti-alkali hemoglobin, glucose-assisted blood and other items.



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